by Larry Coffin
(Halifax, Nova Scotia)
Photo Credit: Matt Allworth
I am in the process of switching our forensic drug screening away from Emit assay with GC/MS confirmation to screening and confirmation by LC/MS/MS. We have always hydrolyzed our urine samples (various methods) and only dealt with total drug levels.
My question is when using the LC/MS/MS with ion trap is it advisable to hydrolyze our urine samples with beta-glucuronidase and then analyze or dilute samples and analyze combining free levels of drug and glucuronide metabolites for the total drug level?